Gruppe Guenat   The Metastasis-on-Chip project aims to replicate the metastatic process, focusing specifically on extravasation and colony formation. Our initial studies evaluate the metastatic potential of cancer cells based on their phenotypes, using the A549 non-small cell lung cancer (NSCLC) cell line, which exhibits distinct phenotypic variations. We discovered that paraclones, characterized by a mesenchymal phenotype, successfully extravasate, while holoclones, with an epithelial phenotype, do not. Additionally, paraclones demonstrated significantly greater migratory behavior compared to holoclones. These findings provide valuable insights into the mechanisms of metastasis and lay the groundwork for further exploration of targeted therapies.